Composite

Part:BBa_K5036038

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-21)


Loading system(CD63, MCP)

Part Description

We've engineered exosomes to carry a specific delivery system which is composed of CD63 and MCP. CD63 is membrane-specific for exosomes while MCP is a specific tagging system for MS2 Protein that helps researchers understand how RNA behaves in living cells and other types of cells.

Usage

In our switch we have loaded our switch with MS2 that has a strong affinity for MCP so When the switch encounters an exosome, the MS2 protein binds to both CD63 and MCP. This interaction causes the switch to be incorporated into the exosome, which is then released into the surrounding environment.

this figure illustrates the structure and function of our loading system. .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 925
    Illegal AgeI site found at 777
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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